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Technická 5
166 28 Prague 6 – Dejvice
IČO: 60461373 / VAT: CZ60461373

Czech Post certified digital mail code: sp4j9ch

Copyright: UCT Prague 2017
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The Department of Water Technology and Environmental Engineering (DWTEE) is a part of the Faculty of Environmental Technology of the University of Chemistry and Technology, Prague. The Department provides education in Czech and English and is active in the research field related to water technology.  Currently, there are approximately 40 master students, 30 PhD. students and 24 staff members at the department. Many of the research projects run at the department are done in cooperation with companies and other research institutions. The department is involved in two International Erasmus Mundus study programmes: The International Master of Science in Environmental Technology and Engineering (IMETE) and the PhD. program Enviromental technologies for contaminated solids, soils and sediments (ETeCoS3).

The Department of Water Technology and Environmental Engineering has 6 informal working groups covering all topics regarding water technology, chemistry, and biology: Anaerobic Technologies, Biological Wastewater Treatment, Hydrobiology and Microbiology, Physical-Chemical Wastewater Treatment, Aquatic Chemistry and Analytics, and Drinking Water Treatment. The research interests of these groups is often shared and they cooperate with each other on scientific work and education. The department cooperates with many institutions and companies from the Czech Republic as well as from other countries.

DWTEE has a long standing history of cooperation with various international professional associations. Professor Vladimir Madera, the Head of the Department at that time, was one of the founding fathers of IAWPRC, the predecessor of International Water Association (IWA). In 1988, Prof. Grau (Head of the Department after Prof. Madera) was elected IWA's Vice-President; in 1990-1994, he served as the Association's President. Members of the DWTEE are active in the following IWA specialist groups:  Anaerobic Digestion; Design, Operation and Costs of Large Wastewater Treatment Plants; Microbial Ecology; and Water Engineering (former Activated Sludge Population Dynamics). DWTEE also cooperates with the European Water Association (EWA), where Professor Jiri Wanner was President between 2005-2007.

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The Department of Water Technology and Environmental Engineering (DWTEE) provides education in both Czech and English. If you are interested in a short, intensive course, Athens is the best choice for you. If you want to spend one or two semesters during your bachelor or masters studies at our Department and you are from an EU country, the Erasmus programme is designed for you. For students from both EU and non-EU countries, there is an alternative option with our Erasmus mundus programmes: Imete or ETeCoS3

The International Master of Science in Environmental Technology and Engineering (IMETE) programme is an International Erasmus Mundus programme and is delivered by a consortium of universities consisting of UCT Prague (Czech Republic), Ghent University (Belgium) and the UNESCO-IHE Institute for Water Education (Delft, Netherlands).

DWTEE participates as an associated partner in the PhD. Erasmus Mundus programme Enviromental technologies for contaminated solids, soils and sediments (ETeCoS3). PhD. students can come with financial support from Erasmus, Erasmus Mundus, or other foundations. ETeCoS3 is provided by a consortium consisting of the University of Cassino (Italy)UNESCO-IHE Institute for Water Education (Netherlands), and Université Paris-Est (France).

If you are interested in our research and would like to cooperate with us or work at our department, feel free to contact the leaders of working groups or the Head of the Department (Prof. Pavel Jeníček).

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The Department of Water Technology and Environmental Engineering (DWTEE) is composed of 6 working groups. Scientific work covers all topics regarding water technology, chemistry, and biology. The working groups often cooperate on scientific work and education. The Department cooperates with many institutions and companies from the Czech Republic as well as from other countries.

 

⇒ Publications

 

⇒ Projects and Grants

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Members of the Department of Water Technology and Environmental Engineering (DWTEE) take part in organizing international study programmes and work in many national and international expert associations. We will be glad to collaborate on research projects within one of the research fields studied at the Department.


→ International Study Programmes

DWTEE cooperates in two Erasmus Mundus international study programmes. For further information, contact Prof. Pavel Jeníček or Dr. Jan Bartáček.

šířka 215pxThe International Master of Science in Environmental Technology and Engineering (IMETE) programme is an International Erasmus Mundus programme. It trains people to apply and develop environmental technologies, offering a wide range of optional study fields in an international environment. IMETE is offered by a consortium consisting of three leading Higher Education Institutes: Ghent University (UGhent, Ghent, Belgium), UNESCO-IHE Institute for Water Education (UNESCO-IHE,Delft, Netherlands), and University of Chemical Technology, Prague (UCT Prague, Czech Republic). Student mobility within Europe is an integral part of the programme, which contains 120 ECTS (2 years). During the programme, students study in Delft (Netherlands), Prague (Czech Republic) and Ghent (Belgium). Master thesis research is carried out in one of these locations or at one of the many associated partner institutions all over the world. Between the first and the second years, summer school is held. More information here.

 

šířka 215px

The Environmental Technology for Contaminated Solids, Soils and Sediments (ETeCoS3)  programme provides education and research at the PhD level, training its doctoral candidates to think globally and co-work in multidisciplinary research teams. It is delivered by three universities: University of Cassino (Italy)UNESCO-IHE Institute for Water Education (Netherlands), and Université Paris-Est (France). The programme is centered around three key topics in environmental pollution: Heavy metals, recalcitrant organic pollutants, and contaminated solids. The programme focuses on fundamental and applied aspects to treat and remove these pollutants as well as on the development of recovery and reuse technologies with market potential. There will be a close connection to practical problems, as e.g. hot spots in the Balkans, brown fields in the Czech Republic, and sediments and soils polluted by mining activities in Minais Gerais (Brazil). UCT Prague (represented by the Department of Water Technology and Environmental Engineering) is an associated partner of the consortium.


→ Expert Associations

šířka 215pxThe Czech Water Association (CzWA) was formed in 2009 from the previous Association of Wastewater Treatment Experts (ACE CR). The CzWA is an independent non-governmental and non-profit organization promoting the sustainable and improved management of the total water cycle. The CzWA is a national member for the Czech Republic in the European Water Association and in the International Water Association.

 

šířka 215pxThe International Water Association (IWA) is a global reference point for water professionals, spanning the continuum between research and practice and covering all facets of the water cycle. Through its network of members and experts in research, practice, regulation, industry, consulting and manufacturing, IWA is in a better position than any other organisation to help water professionals create innovative, pragmatic and sustainable solutions to challenging global needs. Members of DWTEE are active in the following specialist groups of the association:  Anaerobic Digestion; Design, Operation and Costs of Large Wastewater Treatment Plants; Microbial Ecology; and Water Engineering (formerly Activated Sludge Population Dynamics).

šířka 215px

 

The European Water Association (EWA) today represent most European countries. The main goal of the association is to create a forum for the mutual information exchange in all aspects of water pollution control in general and of wastewater collection, treatment and disposal in particular. EWA provides professional service to the European Commission in matters dealing with water pollution control and consultancy of water legislation. The Czech Republic became a member of EWA in May 1997. The activities of EWA in the Czech Republic are organized and co-ordinated by the Czech Water Association (CzWA). Professor Jiri Wanner from DWTEE was EWA President between 2005-2007.

šířka 215pxThe World Toilet Organization (WTO) is a global non-profit organization committed to improving toilet and sanitation conditions worldwide. WTO is also one of the few organizations to focus on toilets instead of water, which receives more attention and resources under the common subject of sanitation. Founded in 2001 with 15 members, it now has 235 member organizations in 58 countries working towards eliminating the toilet taboo and delivering sustainable sanitation. Professor Jiri Wanner is a member of the WTO.

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Department Photogallery

šířka 215px

Department in News

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University of Chemistry and Technology, Prague 

Faculty of Environmental Technology
Department of Water Technology and Environmental Engineering
Technická 5   
166 28 Praha 6 - Dejvice
Czech Republic

Building B, Room No. 116

[ikona] => info [obrazek] => [ogobrazek] => [pozadi] => [obsah] =>

Contacts

Head of Department:

Bartáček Jan, prof.

Scientific Secretary:

Bindzar Jan, Ing., Ph.D.

Treasurer:

Sýkora Vladimír, doc. Ing., CSc.

Librarian:

Šmejkalová Pavla, Dr. Ing.

Administration:

Vymětalová Andrea

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Wastewater can be seen as a source of energy, valuable matter (nitrogen, phosphorus, heavy metals) or pure water. The energy of the organic compounds contained in wastewater and sewage sludges can be recycled using anaerobic technologies, i.e. anaerobic digestion (AD). Biogas (mixture of methane and carbon dioxide), which is the end product of anaerobic digestion, can be further utilized as a source of electricity and heat. The AD process is especially favorable for its low green house gasses production.

Work group Anaerobic Biotechnology deals with optimizing the AD processes and solving some specific problems inherent to AD such as removing (recycling) nitrogen and sulfur compounds released in the AD process. The work group aims to protect natural resources (clean water, fossil energy sources or atmosphere) through employing clean anaerobic technologies.

List of Publications

Projects and Grants

 

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Research areas

Energetically Self-sufficient Wastewater Treatment Plant

The work group Anaerobic Biotechnology has a long-term interest in anaerobic digestion of sewage sludges at wastewater treatment plants (WWTPs). The goal of this effort is energy recovery from sewage sludges in order to cover most of the energy consumption of the WWTP. As an example, the central WWTP in Prague is energetically self-sufficient by almost 90 %. This was achieved by introducing sludge disintegration in thickening centrifuges, introducing thermophilic AD process, improving mixing in digestors and intensive precipitation of the particulate organic matter contained in the incoming wastewater. Applying these measures, we achieved two-fold increase of biogas production at WWTP Prague.

Optimizing anaerobic fermentation of lignocellulosic materials by using anaerobic fungi

The numbers of biogas stations have recently been quickly rising throughout the Europe. In these biogas stations, energy crops, agricultural wastes and other types of organic wastes are transformed to biogas - an alternative source of green energy. Unfortunately, lignocellulosic material (plants and their debris) treated in the biogas stations is not readily degradable: only 40–60 % of the total organic matter can be transformed to methane in the classical AD process. This problem can be solved using anaerobic fungi capable of degradation of cellulosic materials. In nature, these microorganisms occur in rumens of ruminants such as cow or deer, where they help these animals to degrade lignocellulosics. By introducing anaerobic fungi into anaerobic digesters, methane production of the biogas plants can increase by up to 20 %.

H2S removal from biogas

H2S is source SO2 emissions, odour problems and operational problems during burning biogas in cogeneration units. Therefore, it is crucial to remove H2S from biogas. Biological methods for H2S removal from biogas are based on H2S oxidation to elemental sulphur by chemolithotrophic bacteria:

H2S + 0.5O2 --> S0 + H2O

This process can take place in external bioreactor following H2S scrubbing from biogas. It can also be induced inside the anaerobic reactor by introducing small amount of oxygen – so called “microaeration”. Both processes are efficient and our results have shown that microaeration has additional positive effects on the AD process such as better hydrolysis of organic compounds. In general, biological methods for H2S removal are always cheaper and simpler than physical-chemical H2S removal.

Nitrogen removal from liquid effluents from anaerobic digesters

The effluents from anaerobic digesters often contain high loads of ammonium nitrogen. At high concentration, ammonium nitrogen can inhibit the anaerobic digestion (AD) process and, when treated at WWTP, it considerably increases the nitrogen load at the WWTP. Compared to the common nitrification/denitrification process, the nitritation/denitritation process can significantly decrease the consumption of oxygen (by 25 %) and organic substrate (by 40 %) needed for ammonium removal from the liquid phase. Therefore it is intensively studied at our work group. Next to this, we also study methods for nitrogen removal directly from the anaerobic digester, e.g. by sorption on zeolites.

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2017

Capson-Tojo, G., Torres, A., Muñoz, R., Bartacek, J., Jeison, D. 2017 Mesophilic and thermophilic anaerobic digestion of lipid-extracted microalgae N. gaditana for methane production Renewable Energy 105, 539-546

Vital, B., Bartacek, J., Ortega-Bravo, , JC., Jeison, D. 2017 Treatment of acid mine drainage by forward osmosis: Heavy metal rejection and reverse flux of draw solution constituents Chemical Engineering Journal 332, 85-91

Kouba, V., Svehla, P., Catrysse, M., Prochazkova, L., Hrncirova, H., Jenicek, P., Bartacek, J. 2017. How biomass growth mode affects ammonium oxidation start-up and NOB inhibition in partial nitritation of cold and diluted reject water. Environ Technol, ahead of print.

Kouba, V.; Darmal, R.; Vejmelkova, D.; Jenicek, P.; Bartacek, J., Cold shocks of anammox biofilm stimulate nitrogen removal at low temperatures Biotechnol Prog 2017, ahead of print.

Kouba, V., Proksova E., Wiesinger H., Vejmelkova D., Bartacek, J. 2017. Good servant, bad master: Sulfide influence on partial nitritation of sewage. Water Sci Technol, ahead of print.

Kouba, V., Vejmelkova D., Proksova E., Wiesinger H., Concha M., Dolejs P., Hejnic J., Jenicek P., Bartacek, J. 2017. High-rate partial nitritation of municipal wastewater after psychrophilic anaerobic pre-treatment. Environ Sci Technol, ahead of print

Pokorná-Krayzelová, L., Bartáček, J., Vejmelkova, D., Alvarez, A.A., Slukova, P., Prochazka, J., Volcke, E.I.P., Jeníček, P. (2017) “The use of a silicone-based biomembrane for microaerobic H2S removal from biogas.” Separation and Purification Technology 189, pages 145-152. 

Pokorná-Krayzelová, L., Mampaey, K.E., Vannecke, T.P.W., Bartáček, J., Jeníček, P., Volcke, E.I.P. (2017) “Model-based optimization of microaeration for biogas desulfurization in UASB reactors.” Biochemical Engineering Journal 125, pages 171-179.

P. Jeníček, J. Horejš, L. Pokorná-Krayzelová, J. Bindzar, J. Bartáček. Simple biogas desulfurization by microaeration – Full scale experience. Anaerobe (In Press, Accepted Manuscript). DOI: http://dx.doi.org/10.1016/j.anaerobe.2017.01.002

Dolejs, P., Ozcan, O., Bair, R., Ariunbaatar, J., Bartacek, J., Lens, P.N.L., Yeh, D.H. 2017. Effect of psychrophilic temperature shocks on a gas-lift anaerobic membrane bioreactor (Gl-AnMBR) treating synthetic domestic wastewater. Journal of Water Process Engineering, 16, 108-114


2016

Hollinger CH., ..., Jeníček P. a kol. (2016) Towards a standardization of biomethane potential tests. Water Science and Technology 74 (11), 2515-2522.

Pokorna D., Zabranska J., Malik S., Kas J. Effect of corn silage treatment with preservative and fungicide on biogas yield. New Biotechnology 335 (S1-S213),p.S87, 2016

Hejnic Jakub, Dolejs Petr, Kouba Vojtech, Prudilova Andrea, Widiayuningrum Patria and Bartacek Jan. Comparing Anaerobic Treatment of Sewage at 15 °C Using UASB Reactor and Anaerobic Membrane Bioreactor. Environmental Engineering Science. Volume 33, Number 11, doi: 10.1089/ees.2016.0163, ahead of print.

Dolejs Petr, Gotvald Robert, Velazquez Aida M.L., Hejnic Jakub, Jenicek Pavel, and Bartacek Jan. Contact Stabilization with Enhanced Accumulation Process for Energy Recovery from Sewage. Environmental Engineering Science. August 2016, ahead of print. doi:10.1089/ees.2016.0155.

Kouba, V., Widiayuningrum, P., Chovancova, L., Jenicek, P., Bartacek, J. (2016). Applicability of one-stage partial nitritation and anammox in MBBR for anaerobically pre-treated municipal wastewater. Journal of Industrial Microbiology & Biotechnology.

Šmejkalová P., Kužníková V., Merna J., Hermanová S.: Anaerobic digestion of aliphatic polyesters. Water Science & Technology, 73 (10) 2386-2393; DOI: 10.2166/wst.2016.088 (2016).


2015

Krayzelova, L., Bartacek, J., Díaz, I., Jeison, D., Volcke, E.I.P., Jenicek, P. (2015). "Microaeration for hydrogen sulfide removal during anaerobic treatment: a review." Reviews in Environmental Science and Bio/Technology 14(4): 703-725.

Pokorna, D., Zabranska, J. (2015) Sulfur-oxidizing Bacteria in Environmental Technology, Biotechnology Advances, 33, pp. 1246-1259, doi 10.1016/j.biotechadv.2015.02.007

Pokorna D., Carceller J.M., Paclik L., Zabranska J.: Biogas Cleaning by Hydrogen Sulfide Scrubbing and Bio-oxidation of Captured Sulfides. Energy & Fuels (2015), DOI:10.1021/ef502804j

Dolejs P. , Paclik L., Maca J., Pokorna D., Zabranska J., Bartacek J.: Effect of S/N ratio on sulfide removal by autotrophic denitrification. Applied Microbiology and Biotechnology, Vol. 99, Is. 5, pp. 2383-92 , 2015.

Hermanová, S.; Šmejkalová, P.; Merna, J.; Zarevúcka, M.: Biodegradation of waste PET based copolyesters in thermophilic anaerobic sludge, Polym. Degrad. Stabil., 2015, 111, 176-184.

Pacek, L., Svehla, P., Bartacek, J., Radechovsky, J., Hrncirova, H., Shejbalova, S., Balik, J., Jenicek, P. 2015. Direct and indirect effects of oxygen limitation on nitrification process applied to reject water treatment. Desalination and Water Treatment, 56(3), 598-607.

Svehla, P., Radechovsky, J., Hrncirova, H., Pacek, L., Bartacek, J. 2015. Effect of influent nitrogen concentration on feasibility of short-cut nitrification during wastewater treatment in activated sludge systems. Chemical Papers, 69(7), 921-929.

Podzimek, T., Bartacek, J. 2014. Použití jednobuněčných řas jako substrát pro výrobu bioplynu. Bioprospect, 24(4), 101 - 103.


2014

Krayzelova, L., Bartacek, J., Kolesarova, N., Jenicek, P. (2014). "Microaeration for hydrogen sulfide removal in UASB reactor." Bioresource Technology 172(0): 297-302. ISSN: 0960-8524

Dolejš, P., V. Poštulka, Z. Sedláková, V. Jandová, J. Vejražka, E. Esposito, J. C. Jansen and P. Izák (2014). "Simultaneous hydrogen sulphide and carbon dioxide removal from biogas by water–swollen reverse osmosis membrane." Separation and Purification Technology 131: 108-116.

Krayzelova, L., Lynn, T.J., Banihani, Q., Bartacek, J., Jenicek, P., Ergas, S.J. (2014). "A Tire-Sulfur Hybrid Adsorption Denitrification (T-SHAD) process for decentralized wastewater treatment." Water Research 61(0): 191-199. ISSN: 0043-1354.

Jenicek, P., Celis, C.A., Krayzelova, L., Anferova N., Pokorna, D. (2014) Improving products of anaerobic sludge digestion by microaeration. Water Science & Technology, 69 (4) 803-809.

Kouba, V., Catrysse, M., Stryjova, H., Jonatova, I., Volcke, E.I.P., Svehla, P., Bartacek, J. 2014. The impact of influent total ammonium nitrogen concentration on nitrite-oxidizing bacteria inhibition in moving bed biofilm reactor. Water Science and Technology, 69(6), 1227-1233.

Svehla, P., Bartacek, J., Pacek, L., Hrncirova, H., Radechovsky, J., Hanc, A., Jenicek, P. 2014. Inhibition effect of free ammonia and free nitrous acid on nitrite-oxidising bacteria during sludge liquor treatment: Influence of feeding strategy. Chemical Papers, 68(7), 871-878.


2013

Jenicek, P., Kutil, J., Benes, O., Todt, V., Zabranska, J., Dohanyos, M., 2013. Energy self-sufficient sewage wastewater treatment plants: is optimized anaerobic sludge digestion the key? Water Science & Technology, 68 (8) 1739-1744.

Jenicek, P., Celis, C., Picha, A., Pokorna, D. (2013) Influence of Raw Sludge Quality on the Efficiency of Microaerobic Sulfide Removal during Anaerobic Digestion of Sewage Sludge; Journal of Residuals Science & Technology 10, 11-16.

Pokorna D., Maca J., Zabranska J., 2013. Combination of Hydrogen Sulphide Removal from Biogas and Nitrogen Removal from Wastewater. Journal of Residuals Science & Technology 10(1), 41-46.


2012

Bartacek, J., Fermoso, F.G., Vergeldt, F., Gerkema, E., Maca, J., Van As, H., Lens, P.N.L. 2012. The impact of metal transport processes on bioavailability of free and complex metal ions in methanogenic granular sludge. Water Science and Technology, 65(10), 1875-1881.

Jenicek, P., Bartacek, J., Kutil, J., Zabranska, J., Dohanyos, M. 2012. Potentials and limits of anaerobic digestion of sewage sludge: Energy self- sufficient municipal wastewater treatment plant? Water Science and Technology, 66(6), 1277-1281.

Procházka, J., Dolejš, P., Máca, J., Dohányos, M. 2012. Stability and inhibition of anaerobic processes caused by insufficiency or excess of ammonia nitrogen. Applied Microbiology and Biotechnology, 93(1), 439-447.

Procházka, J., Mrázek, J., Štrosová, L., Fliegerová, K., Zábranská, J., Dohányos, M. 2012. Enhanced biogas yield from energy crops with rumen anaerobic fungi. Engineering in Life Sciences, 12(3), 343-351.


2011

Jenicek, P., Celis, C.A., Koubova, J., Pokorna, D. 2011a. Comparison of microbial activity in anaerobic and microaerobic digesters. Water Science and Technology, 63(10), 2244-2249.

Jenicek, P., Celis, C.A., Koubova, J., Ruzickova, I. 2011b. Change of the digested sludge quality at microaerobic digestion. Journal of Residuals Science and Technology, 8(2), 39-44.

Procházka J., Dolejš P., Máca J., Dohányos M. 2011. Stability and inhibition of anaerobic processes caused by insufficiency or excess of ammonia nitrogen, Appl Microbiol Biotechnol, DOI: 10.1007/s00253-011-3625-4.

Raposo, F., Fernández-Cegrí, V., de la Rubia, M.A., Borja, R., Béline, F., Cavinato, C., Demirer, G., Fernández, B., Fernández-Polanco, M., Frigon, J.C., Ganesh, R., Kaparaju, P., Koubova, J., Méndez, R., Menin, G., Peene, A., Scherer, P., Torrijos, M., Uellendahl, H., Wierinck, I., de Wilde, V. 2011. Biochemical methane potential (BMP) of solid organic substrates: Evaluation of anaerobic biodegradability using data from an international interlaboratory study. Journal of Chemical Technology and Biotechnology, 86(8), 1088-1098.


2010

Bartacek, J., Fermoso, F.G., Catena, A.B., Lens, P.N.L. 2010a. Effect of sorption kinetics on nickel toxicity in methanogenic granular sludge. Journal of Hazardous materials, 180(1-3), 289-296.

Bartacek, J., Manconi, I., Sansone, G., Murgia, R., Lens, P.N.L. 2010b. Divalent metal addition restores sulfide-inhibited N2O reduction in Pseudomonas aeruginosa. Nitric Oxide - Biology and Chemistry, 23(2), 101-105.

Fermoso, F.G., Bartacek, J., Manzano, R., van Leeuwen, H.P., Lens, P.N.L. 2010a. Dosing of anaerobic granular sludge bioreactors with cobalt: Impact of cobalt retention on methanogenic activity. Bioresource Technology, 101(24), 9429-9437.

Fermoso, F.G., Bartacek, J., Lens, P.N.L. 2010b. Effect of vitamin B12 pulse addition on the performance of cobalt deprived anaerobic granular sludge bioreactors. Bioresource Technology, 101(14), 5201-5205.

Fliegerová K., Mrázek J., Hoffmann K., Zábranská J., Voigt K. 2010. Diversity of anaerobic fungi within cow manure determined by ITS1 analysis. Folia Microbiologica 55(4), 319-325.

Jenicek, P., Koubova, J., Bindzar, J., Zabranska, J. 2010. Advantages of anaerobic digestion of sludge in microaerobic conditions. Water Science and Technology, 62(2), 427-434.

Kennes, C., Lens, P., Bartacek, J. 2010. Air pollution control. Journal of Chemical Technology and Biotechnology, 85(3), 307-308.

Nabarlatz, D., Vondrysova, J., Jenicek, P., Stüber, F., Font, J., Fortuny, A., Fabregat, A., Bengoa, C. 2010. Hydrolytic enzymes in activated sludge: Extraction of protease and lipase by stirring and ultrasonication. Ultrasonics Sonochemistry, 17(5), 923-931.

Novotná Z., Procházka J., Šimůnek J., Fliegerová K., 2010. Xylanases of Anaerobic Fungus Anaeromyces mucronatus, Folia Microbiol. 55 (4), 363–367.

Raposo, F., Fernández-Cegrí, V., De la Rubia, M.A., Borja, R., Beltrán, J., Cavinato, C., Clinckspoor, M., Demirer, G., Diamadopoulos, E., Frigon, J.C., Koubova, J., Launay, M., Méndez, R., Menin, G., Noguerol, J., Uellehdahl, H., West, S. 2010. Quality improvement in determination of chemical oxygen demand in samples considered difficult to analyze, through participation in proficiency-testing schemes. TrAC - Trends in Analytical Chemistry, 29(9), 1082-1091.

Švehla, P., Jeníček, P., Habart, J., Hanč, A., Balík, J. 2010. Testing selected factors influencing nitrification of sludge water. Testování vlivu vybraných faktorů na průběh nitrifikace kalové vody, Chemické listy 104(5), 343-348.


2009

Angelidaki, I., Alves, M., Bolzonella, D., Borzacconi, L., Campos, J.L., Guwy, A.J., Kalyuzhnyi, S., Jenicek, P., Van Lier, J.B. 2009. Defining the biomethane potential (BMP) of solid organic wastes and energy crops: A proposed protocol for batch assays, Vol. 59, pp. 927-934.

Bartacek, J., Vergeldt, F.J., Gerkema, E., Jenicek, P., Lens, P.N.L., Van As, H. 2009a. Magnetic resonance microscopy of iron transport in methanogenic granules. Journal of Magnetic Resonance, 200(2), 303-312.

Bartacek, J., Lens, P.N.L. 2009. Chalcogen cycle science and technology. Environmental Technology, 30(12), 1227.

Bartacek, J., Kennes, C., Lens, P.N.L. 2009b. Biotechniques for air pollution control (biotechniques 2009). Reviews in Environmental Science and Biotechnology, 8(4), 321-323.

Fermoso, F.G., Bartacek, J., Jansen, S., Lens, P.N.L. 2009. Metal supplementation to UASB bioreactors: from cell-metal interactions to full-scale application. Science of the Total Environment, 407(12), 3652-3667.

Pokorna, E., Postelmans, N., Jenicek, P., Schreurs, S., Carleer, R., Yperman, J. 2009. Study of bio-oils and solids from flash pyrolysis of sewage sludges. Fuel, 88(8), 1344-1350.

Raposo, F., de la Rubia, M.A., Borja, R., Alaiz, M., Beltrán, J., Cavinato, C., Clinckspoor, M., Demirer, G., Diamadopoulos, E., Helmreich, B., Jenicek, P., Martí, N., Méndez, R., Noguerol, J., Pereira, F., Picard, S., Torrijos, M. 2009. An interlaboratory study as useful tool for proficiency testing of chemical oxygen demand measurements using solid substrates and liquid samples with high suspended solid content. Talanta, 80(1), 329-337.


2008

Bartacek, J., Fermoso, F.G., Baldó-Urrutia, A.M., Van Hullebusch, E.D., Lens, P.N.L. 2008. Cobalt toxicity in anaerobic granular sludge: Influence of chemical speciation. Journal of Industrial Microbiology and Biotechnology, 35(11), 1465-1474.

Fermoso, F.G., Collins, G., Bartacek, J., Lens, P.N.L. 2008a. Zinc deprivation of methanol fed anaerobic granular sludge bioreactors. Journal of Industrial Microbiology and Biotechnology, 35(6), 543-557.

Fermoso, F.G., Bartacek, J., Chung, L.C., Lens, P. 2008b. Supplementation of cobalt to UASB reactors by pulse dosing: CoCl2 versus CoEDTA2- pulses. Biochemical Engineering Journal, 42(2), 111-119.

Fermoso, F.G., Collins, G., Bartacek, J., O'Flaherty, V., Lens, P. 2008c. Acidification of methanol-fed anaerobic granular sludge bioreactors by cobalt deprivation: Induction and microbial community dynamics. Biotechnology and Bioengineering, 99(1), 49-58.

Fermoso, F.G., Collins, G., Bartacek, J., O'Flaherty, V., Lens, P. 2008d. Role of nickel in high rate methanol degradation in anaerobic granular sludge bioreactors. Biodegradation, 19(5), 725-737.

Nabarlatz, D., Vondrysova, J., Jenicek, P., Stber, F., Font, J., Fortuny, A., Fabregat, A., Bengoa, C. 2008. Extraction of enzymes from activated sludge. pp. 249-257.

Turečková, J., Prokopová, I., Niklová, P., Šimek, J.A.N., Šmejkalová, P., Keclík, F. 2008. Biodegradable copolyester/starch blends - Preparation, mechanical properties, wettability, biodegradation course. Polimery/Polymers, 53(9), 639-643.


2007

Bartacek, J., Zabranska, J., Lens, P.N.L. 2007. Developments and constraints in fermentative hydrogen production. Biofuels, Bioproducts and Biorefining, 1(3), 201-214.

Švehla, P., Jeníček, P., Habart, J., Hanč, A., Černý, J. 2007. The use of accumulation of nitrite in biological treatment of wastewater. Využití akumulace dusitanů při biologickém čištění odpadních vod, 101(10), 776-781.


2006

Zábranská, J., Dohányos, M., Jeníček, P., Kutil, J. 2006. Disintegration of excess activated sludge - Evaluation and experience of full-scale applications, Vol. 53, pp. 229-236.


2000

Dohányos, M., Zábranská, J., Jeníček, P., Štěpová, J., Kutil, V., Horejš, J. 2000. The intensification of sludge digestion by the disintegration of activated sludge and the thermal conditioning of digested sludge, Vol. 42, pp. 57-64.

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Number Provider Title From To Contact person
TA04020951 TAČR Biologická produkce methanolu z odpadního methanu  2015 2017 J. Bartáček
TH01021100 TAČR Recyklace energie ze splaškových odpadních vod v anaerobních membránových reaktorech v prostředí střední Evropy  2015 2017 J. Bartáček
TA03021413 TAČR Využití biomembránových procesů pro odstraňování sulfanu z bioplynu pomocí biochemické oxidace  2013 2015 P. Jeníček
TA01020798  TAČR Komplexní biotechnologie pro spojené odstraňování sulfanu z bioplynu a nutrientů z odpadních vod na čistírnách odpadních vod, bioplynových stanicích a podobných technologických celcích 2011 2014 D. Pokorná
FR-TI1/327 MPO ČR Vývoj odsiřovacího biofiltru pro čištění bioplynu, doba řešení  2009 2013 J.  Zábranská
MSM 6046137308 MŠMT ČR Studium chemických a biologických procesů pro ochranu životního prostředí 2007 2013 P. Jeníček
ERG-2010-268417 European Comission Biofilms in Bioreactors for Advanced Nitrogen Removal from Wastewater 2010 2013 J. Bartáček
QI92A286 NAZV MZe ČR Zvýšení produkce bioplynu z rostlinné biomasy použitím anaerobních hub 2009 2012 M. Dohányos
FI-IM5/183  MPO ČR Suchá fermentace biomasy a tříděného biodegradabilního odpadu s energetickým vyžitím bioplynu k výrobě elektrické energie 2008 2010 J. Zábranská
MEIF-CT-2006-041896 European Comission Bioavailability of Heavy Metals in Anaerobic Granular Sludge 2008 2010 J. Bartáček
SP/3g4/129/07 MŽP ČR Intenzifikace produkce bioplynu 2007 2009 M. Dohányos
FP6-018525 European Comission Reduction, modification and valorization of sludge 2006 2009 P. Jeníček
MEXT-CT-2003-509567 European Comission Novel Biological Engineering Processes for Heavy Metal Removal and Recovery 2004 2008 J. Bartáček
104/05/2501  GAČR  Studium biologických přeměn sloučenin síry pro využití v technologiích ochrany prostředí 2005 2007 J.  Zábranská
104/05/0798  GAČR  Anaerobní technologie pro zpracování odpadů s vysokými obsahy proteinů 2005 2007 J.  Zábranská
104/03/0408 GAČR  Nitritace a denitritace jako prostředek k racionalizaci biologického odstraňování dusíku z odpadních vod 2003 2005 P. Jeníček
104/03/0119 GAČR  Biomethanizace směsných odpadů a rostlinné biomasy 2003 2005 M. Dohányos
MSM 223200003 MŠMT ČR Studium chemicko-technologických procesů pro ochranu životního prostředí a zpracování paliv 1999 2004 M. Dohányos
QD 1069 NZVA MZe ČR Minimalizace množství produkovaných čistírenských kalů 2001 2004 M. Dohányos
104/00/0867 GAČR Intenzifikace kalového hospodářství a možnosti potlačení pěnění na čistírnách odpadních vod pomocí radiační technologie 2000 2002 P. Jeníček
EP9346 NAZV MZe ČR Hygienizace čistírenských kalů 1999 2001 M. Dohányos
104/96/0449 GAČR Kombinované odstraňování organických látek a nutrientů z odpadních vod anaerobně-aerobním čištěním 1996 1998 P. Jeníček
104/96/0449 GAČR Kombinované odstraňování organických látek a nutrientů z odpadních vod anaerobně-aerobním biologických čištěním 1996 1996 P. Šmejkalová

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The research group called Biological Wastewater Treatment primarily focuses on a large variety of topics related to municipal waste water treatment plants. It deals with the issues of nutrient removal, particularly nitrogen and phosphorus, including various ways of bio-augmentation.


An important part of the work consists in sludge analyses mainly with respect to separation properties of the sludge and the presence of specific metabolic groups of organisms. This also involves molecular biology methods such as FISH or PCR-DGGE. 


The group also engages in forming mathematical models for wastewater treatment plants and proposing optimisation of their management and operation.

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Hydrobiology and microbiology are significant in: assessing the ecological state of steady and running waters, studying eutrophication and acidification, evaluating the state and efficiency of water management and treatment technologies as well as for operations with cooling water.


Microorganisms can indicate both the processes that are in progress and the efficient technological conditions or status but they can also be used for biotechnological purposes. Selected microorganisms are even used in laboratories or semi-operations to detect the efficiency of newly introduced materials, agents and intermediates.


In this respect, biology mainly focuses on applying theoretical knowledge and its utilization to indicate the status of processes. Results of biological analyses should enable a technologist to draw relevant conclusions and take effective measures. What is also important is the legislative framework where the knowledge from biological fields is implemented. The future of biology lies in the application of molecular biology methods (PCR, DGGE etc.). All the methods are applicable both in teaching and laboratory practice. Another advantage is the connection of undemanding microscopic methods with cultivation methods and the methods carried out on a purely molecular-biological material.

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Effects on microclimate, air quality, water and soil ecosystems within the scope of hydric recultivation of brown coal mines.

Extensive hydric recultivation, flooding the residual pit of the Most – Ležáky quarry executed within the scope of revitalising the area affected by mining will significantly improve the countryside characteristics north of the Most city. The aim to recultivate brown coal mines is a subject to the assessment of environmental impacts (EIA). The objective of this assessment is to identify, describe and evaluate the expected effects of the projects being prepared (buildings, activities and technologies). The result of the project will consist of the complex methodology quantifying environmental impacts of brown coal mines recultivation. In accordance with the framework regulation for water management 2000/60/ES the assessment of the local environmental status is performed mainly on the basis of water and periphyton samples (the main components being plankton and benthos).

Importance of drinking water quality degradation occurring in its transport and accumulation

Drinking water will be safe in the whole supply system only if contamination of water resources is prevented, water is treated sufficiently (the pollutants present are removed completely or partially to comply with requirements for quality and safety of drinking water) and also the secondary contamination is prevented during drinking water accumulation and distribution and operations with it. Water tanks and towers as buildings cannot be substituted in the water supply system and they also largely contribute to the reliability of the system, its maintainability or vulnerability. These buildings are strategically significant and can influence the water quality. Their location also greatly affects economical costs of the water supply system and its operation.


Aspects for assessing the impact of water tanks on the quality of the water supplied were solved to define both external and internal factors which crucially influence the water quality and its stability in accumulation tanks as well as in the distribution network. There were identified different levels of importance of partial causes affecting the quality of treated, accumulated and transported drinking water, which was a significant step allowing us to focus on the most effective methods minimizing the formation of biofilms, present biological life in water tanks and towers and to specify the rules for exchange of water in accumulation tanks. A prototype of a filtration device was developed to be placed into venting shafts in water tanks. Technical directive I-D-48 Construction arrangement, operation and maintenance of water tanks was prepared during the project solution and it became the basis for the efficiency verification of the directive of ČSN 73 6650 Water tanks, which applies to the design and operation of water tanks (both of the ground and tower types, which are the components of the water network supplying the public with drinking water).


Currently, the issues related to biologically stable drinking water are being researched further in accordance with Plans for Drinking Water Safe Supply (Water Safety Plans) and hazard analysis (HACCP).

Research of nanosurfaces

This workplace belongs to the NANOPIN research centre. In its biological section it implements the methods testing material surface properties (applied on glass, fabrics, pipelines in contact with water). Currently, a directive is being prepared dealing with testing the antibacterial properties of nanosurfaces. Simple methods testing material surface properties will be then put into practice.
Tested materials will also include filtration materials that are expected to become a part of filters used in water treatment operations.

New materials and technologies for material conservation of historical monuments and preventive monument care.

The project goal is to develop new materials and technologies and to verify their functionality for the following purposes: efficient but gentle removal of unwanted secondary layers from material surfaces, mainly the layers of oils and polymers; consolidation of construction monuments damaged by weathering and impregnating the porous inorganic substrate with innovated solidifying materials; preventive protection of materials forming the monument surface against weathering effects, namely water, and against biodegradation by applying new hydrophobic and biocidic agents. Newly developed materials and technologies will focus on the increased efficiency and care of applications with regard to the materials treated and the environment, prolonged lifetime, reversibility and the quality of processes comprising monument preventive, restoration and conservation actions. The development stage is to implement new knowledge from the basic material research, mainly nanomaterials and nanotechnologies including their combinations with traditional materials and methods. The research focuses on solving the tasks of the topical priority Materials and technologies for protection and preservation of cultural heritage of the NAKI Program.

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The water cycle in nature can be influenced by
he can never control it, but he must always protect it ...


Drinking water occupies a privileged position among the foodstuffs that a person needs for his life. For this reason, it is necessary to pay increased attention to its quality. In simple terms, we could understand this only in relation to the production of drinking water, ie the individual technological steps and procedures that are used in its production and should provide high-quality drinking water with an optimal composition. In relation to the quality of drinking water required by the consumer, however, this issue moves to a much broader level, starting with the protection of water resources, continuing our own technology of drinking water production, but also its distribution to the consumer. Only by controlled professional intervention in these three areas can the desired goal be achieved - quality drinking water. All these areas are the subject of the focus of our working group.

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Hydrochemistry

The chemical behavior of inorganic substances and new PPCPs depends on their chemical structure. A common type of water analysis usually determined the total concentration of individual components. In fact, the individual components occur in various forms of existence (species), which result from protolytic, complex-forming, oxidation-reduction, polymerizing, and biodegrading reactions. It is difficult to determine the individual forms experimentally and so they are frequently calculated on the basis of chemical thermodynamics laws. For this purpose, a range of calculation programs already exists.

Hydroanalytics

Development and extended applications of various new PPCPs require the preparation of suitable analytical methods for their identification and quantification. Among the methods, applicable belong particularly the methods of instrumental analysis allowing detection of the presence of the PPCPs selected even at nanogram concentrations. Attention is primarily paid to selected medicaments, cosmetic preparations, and surfactants. The researchers closely cooperate with the Czech Office for Standards, Metrology, and Testing (ÚNMZ) in creating new regulations ÈSN. They also deal with the assessment and adaptation of new hydro-analytical directives ISO and EN.

Treatment technologies

The main attention is paid to the basic treatment processes of both surface and groundwater. In this context, for example, the optimization of coagulation test conditions is solved in order to obtain both high-quality treated water and a well-separable suspension. In the case of groundwater, it is primarily a solution of the calcium-carbonate balance, which is the dominant balance in the de-acidification, de-ironing, and de-manganization of these waters.

Ion exchange, sorption, and membrane processes

These methods are used for selective and non-selective removal of ions/solutes from water. Particularly attractive is the selective removal of heavy metals from water using ion exchange technologies, the removal of arsenic using inorganic sorbents, or the removal of trace concentrations of organic substances (see below). Membrane technologies can be used not only for the treatment of drinking water but also for the treatment of some types of wastewater.

Modern oxidation processes

The so-called Advanced Oxidation Processes (AOPs) have demonstrated considerable potential in removing difficult-to-biodegrade pollutants from wastewater. Their common denominator is the course at normal temperatures and pressures, and especially the use of extraordinary reactivity of hydroxyl radicals. At present, we are mainly dealing with processes based on the use of ozone and hydrogen peroxide. The main research topics include the possibilities of using AOPs for the removal of nitrogen compounds, with emphasis on the possibility of oxidation of ammoniacal nitrogen to gaseous nitrogen or increasing the biological cleanability of industrial wastewater.

Micropollutants

The issue of water treatment has recently shifted to the field of micro-pollution of water resources, especially metabolites of pesticides, or drug residues, and ATB resistance genes. We deal with the influence of advanced oxidation processes and sorption on various types of materials to remove these substances. By using sorption or precipitation processes, we also solve the removal of heavy metals, we also pay attention to the issue of microplastics.

Biological stability of drinking water

In real distribution systems, we can often notice the presence of residual organic substances after treatment processes designed to reduce the content of organic carbon in water, which is the reason for increasing the production of organic matter, biofilm, which covers the inner walls of pipes or parts of technology. One of the chemical-biochemical methods that evaluate or, as a result, predict the deterioration of drinking water quality is the determination of the BDOC (biodegradable dissolved organic carbon) indicator. The work of the working group is also focused on this method of evaluating the biological stability of water.

Recycling and reuse of treated wastewater

The Group participates in solving the topic of recycling and reuse of treated (urban) wastewater, where it is possible to replace the drinking water used so far with this water. This is mainly water for irrigation in agriculture, lawns of sports facilities (golf, football), urban greenery, or utility water to maintain the cleanliness of cities and towns. The economic intention is to produce service water that is cheaper than drinking water used for these purposes, while at the same time using water resources sparingly. The project of recycling and reuse of treated wastewater combines two relatively different topics of water technology: water treatment and wastewater treatment.

Monitoring the quality of watercourses and rainwater

We have been monitoring the quality of the upper reaches of the Krkonoše rivers for a long time, especially the Elbe, Úpa, Jizera, and Jizerka. At the same time, the quality of rainwater in the area of Luční hora - Strážné is also evaluated. Sampling is performed directly in the flow of the mentioned rivers and flows are measured using a magnetic induction flow meter in order to evaluate the mass balances of the monitored indicators.


Our workplace provides:

  • Abbreviated analysis of drinking water according to Decree No. 252/2004 Coll. as amended
  • Analysis of pool water according to Decree No. 238/2011 Coll. as amended
  • Analyzes of water from heating systems, including evaluation of aggressive or incrusting properties according to TNV 75 71 21
  • Determination of metals by AAS method with flame and electrothermal atomization
  • Determination of TOC, DOC and NPOC in waters according to ČSN EN 1484
  • Determination of BDOC (biodegradable dissolved organic carbon) in aqueous samples
  • Determination of TOC in solid samples according to ČSN EN 13 137
  • Coagulation tests in order to determine the optimal process conditions
  • Aggregation tests in order to determine the proportion of particle size in the treated sample and to optimize the formation of suspension and mixing with respect to the following separation processes
  • Leachate tests of solid materials related to Decree No. 387/2016 Coll.
  • Sorption tests focused on the kinetics of the process, including the determination of the sorption capacity of the respective sorbent in a statistical and dynamic arrangement
  • Determination of adsorbable organically bound halogens (AOX) in solid and liquid samples
  • Determination of extractable organically bound halogens (EOX) in solid samples
  • Tests of wastewater oxidation by ozonation, Fenton reaction, and their modifications, aimed at determining the efficiency of the process and its optimization
  • Tests of anaerobic biodegradability of organic substances (including plastics) according to ČSN EN ISO 11734, ČSN EN ISO 14853
  • design of technology for the elimination of micropollutants (drugs, pesticides, ATB resistance genes, etc.) into drinking and wastewater
     

    For more information contact:

Nina StrnadovaAlena Honova, či Pavla Šmejkalova

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E-mail: Katerina.Demnerova@vscht.cz
Phone.: 3025
Room: Y01a
Fax:  

 

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Prof. Katerina Demnerová is the head of the Laboratory of Food Microbiology in DBM at UCT Prague and the head of Accredited Laboratory  of Microbiology and GMO no. 1316.3 (ISO 17025) in DBM. In 1997 she became professor in Microbiology. She was the Head of DBM at UCT Prague in 2006-2013. Her research activities are focused on environmental and food microbiology and GMO. In these fields she published 226 articles (ISI Web) with citation index 2248 (2003) and the Hirsch index 25 (13.11.2018). She is a member of the international editorial board of journals International Microbiology (ISSN 11396709) and International Biodeterioration and Biodegradation (ISSN 0964-8305). In 10 past years she worked with her team on more than 25 national or international projects, when for some of them she was the leader. She presented more than 250 lectures or posters at international or national conferences. She is an active member of experts group Section of Environmental Biotechnology EFB, European Network of Genetically Modified Microorganisms Laboratories (ENGL, Ispra, Italy), the chair-woman of Technical committee for microbiology in Czech Accreditation Institute, the chairwoman of the organizing committee of the international conference „Biodegradation and Biodeterioration“ and the chairwoman of Section for food microbiology in Czechoslovak Society for Microbiology. She supervises Bc., M.Sc., and Ph.D. theses. She is Senior Lecturer for classes of General Microbiology, Food microbiology, Pharmaceutical microbiology, Food safety, Environmental engineering and others in BSc., MSc. and Ph.D. programme in Czech and English language. In 2011 she obtained Milada Paulová Prize for whole-life scientific research and in 2012 Votočka´s medal awarded by UCT Prague.


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E-mail: Sabina.Purkrtova@vscht.cz
Phone.: 5204
Room: Y01b 
Fax:  

 

[ikona] => [obrazek] => 0002~~Cw4AAA.jpg [obsah] =>

Dr. Sabina Purkrtová  is a research specialist since 2007 and assistant professor since 2015 in the Laboratory of Food Microbiology in the DBM in UCT Prague since 2007. In 2014 she finished her Ph.D. in study programme Microbiology (with dissertation thesis: Application of modern methods for study of food microorganisms). Her research and pedagogic work in the field of food and forensics microbiology is focused on the following main specialisations: the detection, enumeration and identification of food-borne pathogens and other microflora by the cultivation and rapid methods (including PCR applications, RT-PCR applications,  sequencing, MALDI-TOF MS and immunochemical methods), genotyping and phenotyping methods, the characterisation of food-borne microorganisms for their antibiotics resistance, biofilm formation and biofilm resistance to antimicrobial agents. In these fields she supervised or consulted more than 25 bachelor and 20 diploma thesis. She is a lecturer for classes and laboratory practice in Food microbiology for BSc. and MSc. programme in Czech and English language. She also organises and lectures theoretical and practical courses in food microbiology for national and international counterparts from  food industry and control laboratories. She published 13 articles (ISI Web) with citation index 32 (29) and the Hirsch index 4 (13.11.2018). She presented more than 20 posters or lectures at international or national conferences. She has been a team-member in more than 10 national and international research projects in food microbiology. She is a team-member in Accredited Laboratory of Microbiology and GMO no. 1316.3 (ISO 17025) in DBM as the specialist for metrology and MALDI-TOF MS method. In 2010 she passed five months study stay in University of Bologna at Department of Food Science.


Publications in impacted journals

  • Pachlová V., Buňková L., Purkrtová S., Němečková I., Havlíková Š., Purevdorj K., Buňka F. Contaminating microorganisms in quark‐type cheese and their capability of biogenic amine production. Int. J. Dairy Technol. 2018, 71, 1018-1022.
  • Kraková L., Šoltys K., Otlewska A., Pietrzak K., Purkrtová S., Savická D., Puškárová A., Bučková M., Szemes T., Budiš J., Demnerová K., Gutarowska B., Pangallo D. Comparison of methods for identification of microbial communities in book collections: Culture-dependent (sequencing and MALDI-TOF MS) and culture-independent (Illumina MiSeq). Int Biodeterior Biodegradation. 2018, 131, 51-59.
  • Zdeňková K., Alibayov B., Karamonová L., Purkrtová S., Karpíšková R., Demnerová K. Transcriptomic and metabolic responses of Staphylococcus aureus in mixed culture with Lactobacillus plantarum, Streptococcus thermophilus and Enterococcus durans in milk. J. Ind. Microbiol. Biotechnol. 2016, 43, 1237-1247.
  • Pachlová V., Buňka F., Buňková L., Purkrtová S., Havlíková Š., Němečková I. Biogenic amines and their producers in Akawi white cheese. Int. J. Dairy Technol. 2016, 69, 386-392.
  • Vondráková L., Purkrtová S., Pazlarová J., Demnerová K. Species differentiation of thermotolerant Campylobacters based on distinctive banding patterns obtained by multiplex PCR. Czech. J. Food Sci. 2015, 33, 27–31.
  • Laknerová I., Zdeňková K., Purkrtová S., Piknová Ľ., Vyroubalová Š., Hanák P. Interlaboratory identification of Black seabream (Spondyliosoma cantharus) as a model species on basis of PCR targeting the second intron of the parvalbumin gene. J. Food Qual. 2014, 37, 429-436.
  • Alibayov B., Zdeňková K., Purkrtová S., Demnerová K., Karpíšková R. Detection of some phenotypic a genotypic characteristics of Staphylococcus aureus isolated from food items in the Czech Republic. Ann. Microbiol. 2014, doi 10.1007/s13213-014-0802-6.
  • Pazlarová J., Purkrtová S., Babuliková J., Demnerová K. Effect of ampicillin a vancomycin on Staphylococcus aureus biofilms. Czech. J. Food Sci. 2014, 32, 137-144.
  • Složilová I., Purkrtová S., Kosová M., Mihulová M., Šviráková E., Demnerová K. Antilisterial activity of lactic acid bacteria against Listeria monocytogenes strains originating from different sources. Czech. J. Food Sci. 2014, 32, 145-151.
  • Purkrtová S., Turoňová H., Pilchová T., Demnerová K., Pazlarová J. Resistance of Listeria monocytogenes biofilms to disinfectans. Czech. J. Food Sci. 2010, 28, 326-332.
  • Purkrtová S., Babulíková J., Demnerová K., Pazlarová J. Antimicrobial factors effect on biofilm formation in Staphylococcus aureus. Czech. J. Food Sci. 2011, 29, S1-S10.

Experience with R&D Projects

  • DG18P02OVV062 Biodiversity of black and white photografic and cinematografic materials in the czech archives and the methods of their desinfection (2018-2022)
  • GACR 17-15936S Interaction of nanoparticles modified by natural compounds with biofilms of pathogenic microorganisms (2017-2020)
  • LD14097 Mechanisms of bacterial attachment/detachment, survival and biofilm formation on the surfaces. Stress response vs. resistance development and persistence (2014-2016)
  • GACR 14-23597S Impact of environmental factors on biofilm detachment (2014-2016)
  • MZE QJ1210300 Protection systems of quality and safety of dairy products by means of suitable methods applicable in practice (2012-2016) 
  • LD11048 Genetic backround of ATB resistance in Salmonella spp. isolates from Prague wastewater treatment plant (WWTP) (2011-2012)
  • 2B08074 Methods of evaluation of hygiene standards and cleaning effectivity used for process equipment and environment in dairy plants, procedures of detection and removal persistant strains as tools of milk processing control into high-quality and safety foods (2008-2011)
  • 2B08050 Listeria monocytogenes - processes enabling reliable appreciation of quality and safety of dairy products, phases of production technological process, final products and their storage (2008-2011)
  • BIOTRACER (6th RTD Framework) - Improved biotraceability of unintended microorganisms and their substances in food and feed chains (2007-2010)
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E-mail: Kamila.Zdenkova@vscht.cz
Phone.: 5196
Room: Y02
Fax:  

 

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My name is Kamila Zdeňková and I am a research specialist at UCT Prague since 1999 and assistant professor since 2007 in the Laboratory of Food Microbiology. As an undergraduate student, I joined the group of prof. Kateřina Demnerová. I started to work in her laboratory in 1996. From the beginning I focused on analysis of pathogens in foodstuff with the special emphasis on the bacteria of genus Listeria. Initially I used various classical microbiology techniques, later fast and specific identification of genome of interest. In 1999 I started my Ph.D., the topic of my thesis was “Detection of genetically modified organisms (GMO) in food using the molecular biology methods”.


Selected publications in impacted journals

  • Zdeňková K., Akhatova D., Fialová E., Krupa O., Kubica L., Lencová S., Demnerová K.: Detection of meat adulteration: Use of efficient and routine-suited multiplex polymerase chain reaction-based methods for species authentication and quantification in meat products. Journal of Food and Nutrition Research, Volume 57 (4), 351-362 (2018). WOS:000451926900004.
  • Debode F., Zdenkova K., Janssen E., Tizolova A., Du Jardin P., Berben G., Demnerova K.: Development of real-time PCR assays for the detection of the pin II terminator (tpinII) used in GM constructs and its donor organism, potato (Solanum tuberosum). Food Analytical methods, 11: (8), 2172-2180 (2018). DOI: 10.1007/s12161-018-1203-4.
  • Boháčová M., Zdeňková K., Tomáštíková Z., Fuchsová V., Demnerová K., Karpíšková R., Pazlarová J.: Monitoring of resistance genes in Listeria monocytogenes isolates and their presence in the extracellular DNA of biofilms: a case study from the Czech Republic. Folia Microbiologica, Volume 63 (5), 653-664 (2018). DOI: 10.1007/s12223-018-0603-6.
  • Akhatova D., Laknerová I., Zdeňková K., Ólafsdóttir G., Magnúsdóttir S., Piknová L., Kýrová V., Lerch Z., Hanák P.: International interlaboratory study on TaqMan real-time polymerase chain reaction authentication of black seabream (Spondyliosoma cantharus). Journal of Food and Nutrition Research, 57: (1), 27-37 (2018). WOS:000430630000003.
  • Zdenkova K., Alibayov B., Karamonova L., Purkrtova S., Karpiskova R., Demnerova K.: Transcriptomic and metabolic responses of Staphylococcus aureus in mixed culture with Lactobacillus plantarum, Streptococcus thermophilus and Enterococcus durans in milk, Journal of Industrial Microbiology and Biotechnology 43 (9), 1237-1247 (2016). DOI: 10.1007/s10295-016-1794-y.
  • Laknerová I., Zdeňková K., Purkrtová S., Piknová Ľ., Vyroubalová Š., Hanák P.: Interlaboratory identification of Black seabream (Spondyliosoma cantharus) as a model species on basis of PCR targeting the second intron of the parvalbumin gene. J. Food Qual. 2014, 37, 429-436.
  • Valihrach L., Alibayov B., Zdenkova K., Demnerova K.: Expression and production of staphylococcal enterotoxin C is substantially reduced in milk. Food Microbiol. 44, 54-59 (2014). DOI: 10.1016/j.fm.2014.05.020.
  • Alibayov B., Baba-Moussa L., Sina H., Zdeňková K., Demnerová K.: Staphylococcus aureus mobile genetic elements, Mol Biol Rep, 5005-5018, 2014. DOI: 10.1007/s11033-014-3367-3.
  • Alibayov B., Zdeňková K., Purkrtová S., Demnerová K., Karpíšková R.: Detection of some phenotypic and genotypic characteristics of Staphylococcus aureus isolated from food items in the Czech Republic, Annals of Microbiology. Ann Microbiol, 1587-1596 (2014). DOI: 10.1007/s13213-014-0802-6.
  • Alibayov B., Karamonová L., Hollerova R., Zdenkova K., Demnerova K.: Differences in transcription and expression of staphylococcal enterotoxin C in processed meat products, LWT - Food Science and Technology, 64 (2), 578-585 (2015). DOI: 10.1016/j.lwt.2015.06.026.

Experience with R&D Projects

  • MZe (NAZV) QK1910231: New approaches for the proof of fish meat adulteration using genomic DNA, 2019-2023; UCT coordinator.
  • GA17-15936S: Interaction of nanoparticles modified by natural substance with biofilms of pathogenic microorganisms, 2017-2019; team member (researcher).
  • MPO, OP PIK APLIKACE, CZ.01.1.02/0.0/0.0/16_084/0009936: Aplikace nanovláken v potravinářských obalech, 2017-2020; team member (researcher).
  • MZe (NAZV) QK1720263: Diagnostic methods for laboratory control of Papaver L. authenticity, 2017- 2019; team member (researcher).
  • ESF, OP VVV, CZ.02.2.69/0.0/0.0/16_018/0002732, Modernizace stávajících a tvorba doktorských studijních programů na VŠCHT Praha, 2014-2020; pedagogue.
  • MZe (NAZV) QJ1530272: Complex strategies for effective detection of food fraud in the chain productionconsumer, 2015-2018; team member (researcher).
  • Projects of Internal Grant Agency of UCT Prague, e. g. C_VSCHT_2015_031, IGS VSCHT or C1_VSCHT_2017_018; team member (researcher).
  • TRIGGER, FP7, SP4 CAPACITIES, SCIENCE-IN-SOCIETY-2013, EUROPEAN COMMISSION, DG R&I, Grant Agreement 6110342014-2017; team member (expert collaborator).
  • LE14016 PEDICEV program EURPO II, MŠMT, 2014-2017; team member (expert collaborator).
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E-mail:  
Phone.:  
Room:  
Fax:  
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Ing. Viviana Fuchsova is a student of the third year of doctoral studies at Institute of Chemical Technologies, Prague majoring in Microbiology. In solving her dissertation thesis she focuses mainly with the characterization of multi-species biofilms of significant bacterial pathogens. In the course of study, she deals with the testing of bacterial resistance to various antibiotic substances (from the group of β-lactams, quinolones, glycopeptides and aminoglycosides). Further she also pursues its inter-species transfer through monitoring the presence of sequential parts of the resistance genes. Her Ph.D. thesis supervisor is prof. Kateřina Demnerová.


Publications

  • Bohacova M., Zdenkova K., Tomastikova Z., Fuchsova V., Demnerova K., Karpiskova R., Pazlarova J. 2018. Monitoring of resistance genes in Listeria monocytogenes isolates and their presence in the extracellular DNA of biofilms: a case study from the Czech Republic. Folia Microbiol. 63(5):653-664.
  • Fuchsová V., Zdeňková, K., Demnerová, K. 2017. Současný pohled na antibiotické rezistence. Bioprospect. 27(2): 33-39.
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E-mail: Ekaterina.Shagieva@vscht.cz
Phone.: 5198
Room: Y03
Fax:  

 

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Mgr. Ekaterina Shagieva – a student in Ph.D. degree programme “Microgiology (in the 3rd year), which focuses on the problematics of Campylobacter spp. including their ATB resistance, subject of Ph.D. thesis:  The role of environmental factors in survival of Campylobacter jejuni in suboptimal conditions.Her Ph.D. thesis supervisor is prof. Kateřina Demnerová.

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Bc. Milada Suková – a student in master degree programme “Microbiology” (in the 2nd final year), which studies the problematics of the detection ATB resistance by new methods, subject of diploma thesis: Application of MALDI-TOF MS and PCR for the detection of Salmonella spp. resistance to beta-lactam antibiotics. Her master thesis supervisor is dr. Sabina Purkrtová.

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