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Thesis topic:

Study of the properties and inhibitory effects of metal nanoparticles on organisms

Cooperation with Institute of Chemical Technology of Monuments and Academy of Sciences of the Czech Republic


Biodeterioration (biogeophysic and biogeochemical) is known as unwanted change in material properties (buildings, etc.) caused by the activity of living organisms, such as cyanobacteria, algae, lichens, bryophytes (or bacteria and fungi). These organisms in most cases aesthetically damaging buildings (vegetation stained) and is functionally degrade. For understanding of the biological diversity of microorganisms is necessary to know the relationship between microorganisms and substrate. Knowledge of microbial populations colonizing habitats is fundamental to successful intervention against their propagation, or assumption of successful conservation practices, including their controls.


Newly developed materials and technologies will be focused on achieving increased efficiency and friendliness to material and environment friendly, long life, reversibility and process quality preventive conservation, restoration and conservation activities. Their development will benefit from the new findings of basic research on materials, especially nanomaterials and nanotechnology, including the combination with the now traditional materials and technologies. The aim of the work is inhibitory toxicity tests on selected populations of organisms that are involved in biodeterioration of surfaces cultural monuments, buildings and collection of exhibits. Focusing in particular on the biocidal formulations containing nanoparticles of metals (various sizes and concentrations). Selected biocidal products will be tested according to ISO 8692 and ISO 10 260. From the results obtained, the proposed methodology suitable testing special types of surfaces and methods for their treatment. The work is part of the project solved NAKI No. DF11P01OVV012


  • High school: Gymnázium Botičská
  • Magister degree VŠCHT Prague, Technology of water, diploma thesis 2012 

Participation on teaching

  • Microbiology and Hydrobiology, Laboratory of hydrobiology and microbiology
  • Bc. Vladimíra Škopová, consultant of diploma thesis: Biocidal effect of metal nanoparticles(2012/2013)
  • Bc. Kateryna Ovcharenko, consultant of diploma thesis: Toxic effect of heavy metals on algal biomass (2012/2013)


  • Zouzelka R., Cihakova P., Rihova Ambrozova J., Rathousky J. (2016): Combined biocidal action of silver nanoparticles and ions against Chlorococcales (Scenedesmus quadricauda, Chlorella vulgaris) and filamentous algae (Klebsormidium sp.). Environ Sci Pollut Res 23:8317–8326.
  • Adámková P., Říhová Ambrožová, J., Říha J., 2012: Využití kovových částic stříbra k potlačování růstu sinic a řas. Sbor. konf. Vodárenská biologie 2012, Praha 1.-2.2. 2012, s. 138-140, ISBN 978-80-86832-65-4
  • Říhová Ambrožová, J., Říha J., Karásková, M., Adámková, P. 2012: Ošetření povrchů nátěry využívající nanotechnologii. Sbor. konf. Voda Zlín 2012, Zlín 14.-15.3. 2012, s. 183-187, ISBN 978-80-86832-65-4
  • Adámková P., Říhová Ambrožová J. 2012: Biocidní účinnost prostředků na bázi nanočástic stříbra. Sbor. sem. Nanomateriály v památkové péči, Národní technické muzeum, 17.5.2012, STOP 2012, 44-52
  • Adámková P., Říhová Ambrožová J. 2012: Biocidní účinnost prostředků na bázi nanočástic stříbra.Zpravodaj STOP, svazek 14, No. 3 (2012), 29-37, ISSN 1212-4168
  • Adámková P., Říhová Ambrožová, J., 2013: Inhibiční účinky nanočástic kovů na kulturu řas. Sbor. konf.Vodárenská biologie 2013, Praha 6.-7.2. 2013, s. 106-111, ISBN 978-80-86832-70-8


Updated: 13.12.2018 15:23, Author: Lucie Pokorná


Doc. RNDr. Jana Říhová Ambrožová, Ph.D.

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